PhD position in Stem Cell Biology of Skeletal Muscle in Zürich

100%, Zurich, temporary

A PhD position in stem cell biology is available in the Laboratory of Regenerative and Movement Biology at the Department of Health Sciences and Technology (D-HEST).

The primary scientific interest and direction of the lab is to study the regenerative and therapeutic potential of stem cells, with emphasis on pluripotent and skeletal muscle cells. Specifically, our group is dedicated to developing stem cell-based therapeutic approaches to treat muscle-associated diseases. To this end, we previously devised a method to reprogram mouse fibroblasts into “induced myogenic progenitor cells” by transient expression of the myogenic transcription factor MyoD in concert with three small molecules. These progenitors can propagate extensively in vitro, and repair disease-afflicted muscles in vivo upon engraftment.

The goal of the proposed research project is to assess which of the myogenic regulatory transcription factors besides MyoD could elicit direct cellular conversion of mouse fibroblasts into induced myogenic stem cells, and further characterize the induced cells molecularly and functionally. The work will involve extensive use of cellular reprogramming methods, intramuscular stem cell transplantation and tissue culture techniques.

Interested applicants should have a master’s degree in Biology, Biotechnology, Genetics, Biochemistry, or an equivalent research related field in addition to a strong background in molecular biology assays. Ideally, these will include experience with mammalian cell culture techniques, gene delivery by retro or lenti-viruses, PCR, qPCR, immunostaining, Western blot and FACS analysis. Experience in stem cell or skeletal muscle biology is preferred, however not essential. Prospective candidates will be part of an international research team and expected to be highly motivated, passionate about science, and collaborative.

ETH Zurich is one of the world’s leading universities specialising in science and technology. We are renowned for our excellent education, cutting-edge fundamental research and direct transfer of new knowledge into society. Over 30,000 people from more than 120 countries find our university to be a place that promotes independent thinking and an environment that inspires excellence. Located in the heart of Europe, yet forging connections all over the world, we work together to develop solutions for the global challenges of today and tomorrow.